Quantification of adipocyte numbers in transgenic mice via the Cre-LoxP recombination sites
Summary: This protocol describes a method to assess adipocyte numbers within a specific depot based on their inducible genomic label.By extracting DNA from dynavap o rings a complete adipose tissue depot stemming from two transgenic mouse lines (Adipoq-CreERT2 x ROSA26-tdRFP and Ucp1-CreERT2 x ROSA26-tdRFP), the number of adipocytes can be determin